Western Blot Positive WB detected in: Jurkat whole cell lysate, 293T whole cell lysate, HL60 whole cell lysate All lanes: CBFA2T3 antibody at 5µg/ml Secondary Goat polyclonal to rabbit IgG at 1/50000 dilution Predicted band size: 72, 63, 64, 8 kDa Observed band size: 72 kDa
IHC image of CSB-PA004576LA01HU diluted at 1:300 and staining in paraffin-embedded human spleen tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.
Supplier Page from CUSABIO Technology LLC for CBFA2T3 Antibody